师资名录

魏娟

长聘教轨副教授

 

邮箱:wwwjuan@sjtu.edu.cn
主页:https://www.x-mol.com/groups/weijuan?lang=en

魏娟博士,1929cc威尼斯长聘教轨副教授、博士生导师。本科毕业于武汉大学,博士师从英国华威大学的高分辨质谱专家Peter O’ Connor教授。回国前在波士顿大学生物医药质谱中心(Center for Biomedical Mass Spectrometry)担任研究科学家。
药物分析,生物多糖和糖蛋白研究
1. 蛋白质糖基化精准分析方法开发
2. 生物多糖和糖蛋白药物分析
3. 基于蛋白质糖基化的生物标记物研究
1. Lu J., Tursumamat N., Liu S., Liu Q., Li H., Su X., Wu S., Guo S., Shen X., Peng W., Wang P., # Wei J. # Programmable One-Tube Sialic Acid Derivatization Enables Dual-Mode N-Glycomics of Low-Input Samples. Analytical Chemistry, 2026. https://doi.org/10.1021/acs.analchem.6c02757
2. Tursumamat N., Liu Q., Lu J. Guo S., Du S., Liu S., Wu S., Li H., Wu P., Li B., # Wei J. # High-sensitive glycomics using seGRAP-mass spectrometry uncovers a conserved N-glycome in single human oocytes. Nature Communications,2026, 17, 5836. (Featured in Editors' Highlights webpage)
3. Guo S., Tursumamat N., Liu S., Liu Q., Wang Z., Lu H., Han Y., Hao P., Zhu J., Wei J. One-Pot O-Glycan Release and Permethylation (OPORP) Enables Rapid and Quantitative O-Glycan Analysis from Low-Input Samples. Analytical Chemistry, 2026, 98, 10: 7613-7622
4. Wu S., Lu J., Tursumamat N., Liu Q., Liu S., Bian Y., Klein J., Han Z., Zaia J., Lin C., Zhu J.,# Wei J.# Unveiling the Accurate Site-Specific N- and O-Glycosylation of Hyperglycosylated Erythropoietin Drugs by an Integrated Approach. Analytical Chemistry, 2025, 97:15410-15419.
5. Liu Q., Lu X., Deng Y., Zhang H., Wei R., Li H., Feng Y., Wei J., Ma F., Zhang Y., Zou X. Global characterization of mouse testis O-glycoproteome landscape during spermatogenesis. Nature Communications, 2025,16(1):2676.
6. Lu J., Guo S., Liu Q., Tursumamat N., Liu S., Wu S., Li H., Wei J. Recent advances in analytical methods and bioinformatic tools for quantitative glycomics. Analytical and Bioanalytical Chemistry, 2025, 13:1-13.
7. Hong P., Xia C., Tang Y., Wei J., Lin C. Glycan mixture analysis by kernel component composition for matrix factorization. Analytical and Bioanalytical Chemistry, 2025, 12:1-10.
8. Li R., Xia C., Wu S., Downs M. J., Tong H., Tursumamat N., Zaia J., Costello C. E., Lin C.,# Wei J.# Direct and Detailed Site-specific Glycopeptide Characterization by Higher-energy Electron-activated Dissociation Tandem Mass Spectrometry. Analytical Chemistry, 2024, 96:1251-1258
9. Wei J.,# Papanastasiou D., Kosmopoulou M., Smyrnakis T., Hong P., Tursumamat N, Klein J., Xia C. S., Tang Y., Zaia, J. Costello, C. E., Lin, C.# De Novo Glycan Sequencing by Electronic Excitation Dissociation MS2-Guided MS3 Analysis on an Omnitrap-Orbitrap Hybrid Instrument. Chemical Science, 2023, 14:6695-6704
10. Chen Z., Wei J., Tang Y., Lin C., Costello C. E., Hong P. GlycoDeNovo2: An Improved MS/MS-Based De Novo Glycan Topology Reconstruction Algorithm. Journal of the American Society for Mass Spectrometry, 2022, 33(3):436-445

Before SJTU
11. Wei J., Tang Y, Ridgeway M. E., Park M. A., Costello C. E., Lin C. Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry Analytical Chemistry, 2020, 92 (19):13211-13220
12. Wei J., Tang Y., Bai, Y., Zaia, J., Costello, C. E., Lin, C. Toward Automatic and Comprehensive Glycan Characterization by Online PGC-LC-EED MS/MS. Analytical Chemistry, 2020, 92(1):782-791
13. Lam Y. P., Wootton C. A., Hands-Portman I., Wei J., Chiu C. K., Romero-Canelon I., Lermyte F., Barrow M. P., O’Connor, P. B. Determination of the Aggregate Binding Site of Amyloid Protofibrils Using Electron Capture Dissociation Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2020, 5;31(2):267-276
14. Wei J., Wu J., Tang Y., Ridgeway M. E., Park M. A., Costello C. E., Zaia J., Lin C. Characterization and Quantification of Highly Sulfated Glycosaminoglycan Isomers by Gated-TIMS NETD MS/MS. Analytical Chemistry, 2019, 91(4), 2994-3001
15. Wu J., Wei J., Hogan J.D., Boons G.J., Lin C. and Zaia J. Sequencing Heparan Sulfate Using HILIC LC-NETD-MS/MS. Analytical Chemistry, 2019, 91, 18, 11738-11746
16. Tang Y.,‡ Wei J.,‡ Costello C. E., Lin C. Characterization of Isomeric Glycans by Reversed Phase Liquid Chromatography-Electronic Excitation Dissociation Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2018, 29(6):1295–1307. (Co-first authors)
17. Wu J., Wei J., Hogan J. D., Chopra P., Joshi A., Lu W., Zaia J. Negative Electron Transfer Dissociation Sequencing of 3-O-Sulfation-Containing Heparan Sulfate Oligosaccharides. Journal of the American Society for Mass Spectrometry, 2018, 29: 1262-1272
18. Lam, Y. P., Wootton, C. A., Hands-Portman, I., Wei J., Chiu C. K., Romero-Canelon I., Lermyte F., Barrow M. P., O’Connor P. B. Does Deamidation of Islet Amyloid Polypeptide Accelerate Amyloid Fibril Formation? Chemical Communications, 2018, 54(98), 13853-13856
19. Khatri K., Pu Y., Klein J. A., Wei J., Costello C. E., Lin C., Zaia J. Comparison of Collisional and Electron-based Dissociation Modes for Middle-down Analysis of Multiply Glycosylated Peptides. Journal of the American Society for Mass Spectrometry, 2018, 29: 1075-1083
20. Maqueda A. S., Xavier G-P, Wei J., Sze S. K. In-depth Analysis of Glutamine Deamidation by LERLIC-MS/MS in Shotgun Proteomics. Analytical Chemistry, 2016, 88(21): 10573-10582
21. van Agthoven M. A., Wootton C. A., Chiron L., Coutouly M. A., Soulby A., Wei J., Barrow M. P., Delsuc M-A, Christian-Rolando C., O’Connor P. B. Two-Dimensional Mass Spectrometry for Proteomics, a Comparative Study with Cytochrome C. Analytical Chemistry, 2016, 88(8): 4409-4417
22. Wei J., Antzutkin O. N., Barrow M. P., Dupree R., Brown S. P., O'Connor P. B. Amyloid Hydrogen Bonding Polymorphism Evaluated by 15N{17O}REAPDOR Solid-state NMR and Ultra-high Resolution FTICR-MS. Biochemistry, 2016, 55(14): 2065-2068
23. Wei J, O'Connor P. B. Extensive Fragmentation of Pheophytin-a by Infrared Multiphoton Dissociation Tandem Mass Spectrometry. Rapid Communications in Mass Spectrometry, 2015, 29(24): 2411-2418
24. Wei J., Bristow A. W. T., O'Connor P. B. The Competitive Influence of Li+, Na+, K+, Ag+, and H+ on the Fragmentation of a PEGylated Polymeric Excipient. Journal of the American Society for Mass Spectrometry, 2015, 26: 166-173
25. van Agthoven M. A., Barrow M. P., Chiron L., Coutouly M. Wootton C. A., Wei J., A., Soulby A., Delsuc M-A, Christian-Rolando C., O’Connor P. B Differentiating Fragmentation Pathways of Cholesterol by Two-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2015, 25: 2105-2114
26. Wei J., Bristow A. W. T., McBride E., Kilgour D. P. A., O'Connor P. B. d-α-tocopheryl Polyethylene Glycol 1000 Succinate: A View from FTICR MS and Tandem MS. Analytical Chemistry, 2014, 86(3):1567-1574
27. Wei J., Li H. L., Barrow M., O’Connor P. B. Structural Characterization of Chlorophyll-a by High-Resolution Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 2013, 24: 753-760
28. Wei J., Gu X., Wang Y., Wu Y, Yan C. Two-dimensional Separation System by on-line Hyphenation of Capillary Isoelectric Focusing with Pressurized Capillary Electrochromatography for Peptide and Protein Mapping. Electrophoresis, 2011, 32(2):230-237
29. Wei J., Gu X., Wang Y., Yan C. Off-line two-dimensional separation system cIEF/pCEC for peptides separation. Chinese Journal of Analytical Chemistry, 2011, 39(2):45-51
30. Mei J., Tian Y. P., He W., Xiao Y. X., Wei J., Feng Y. Q. Preparation approaches of the coated capillaries with liposomes in capillary Electrophoresis. Journal of Chromatography A, 2010, 1217:6979-6986
31. Wu Y., Zhang X. H., Wei J., Xue Y., Bahatibieke M., Wang Y., Yan C. Recent advances and applications of CEC and pCEC. Chinese Journal of Chromatography, 2009, 27(5):609-620
• 痕量样品高灵敏糖基化表征
围绕痕量生物样品中糖链解析的关键难点,分别建立了适用于 N-糖与 O-糖的高灵敏糖组学表征技术体系。发展了溶液增强糖链还原与全甲基化方法(seGRAP),可实现低纳克级蛋白、皮升级血浆及单个人卵细胞的 N-糖组分析,并揭示人卵细胞在个体与发育阶段间高度保守的 N-糖组特征,研究工作被选为Nature Communications Editors’ Highlights亮点文章。针对O-糖的分析挑战,建立一锅法 O-糖链释放与全甲基化策略(OPORP),实现纳克水平糖蛋白和数千细胞的快速、定量 O-糖链分析。此外通过流程化整合,针对同一痕量样品,实现糖链唾液酸连接特异性和唾液酸连接非特异性的双模式解析。相关方法简便,高效,显著降低了糖组学样品需求和操作损失,为痕量样本及深度糖组学研究提供了高灵敏、可推广的分析工具。(Nat. Commun. 2026, 17: 5836;Anal. Chem. 2026, 98(10): 7613-7622;Anal. Chem. 2026, DOI: 10.1021/acs.analchem.6c02757)

• 精准糖基化结构解析
针对糖链结构异构体众多、连接方式复杂及位点微观异质性显著等挑战,发展了融合多维色谱分离、离子淌度、化学衍生化及电子激发解离质谱的精准结构解析策略,以实现糖链组成、分支结构、连接异构体等精细结构的准确表征。在此基础上,进一步建立覆盖游离N-/O-糖链、位点特异性糖肽及完整糖蛋白的多层次分析体系,实现高度糖基化蛋白药物糖基化位点、占有率及位点特异性糖型的系统表征。(Anal. Chem. 2025, 97(28): 15410-15419;Anal. Chem. 2024, 96:1251-1258;Chem. Sci., 2023, 14:6695-6704;Anal. Chem. 2020, 92(19): 13211-13220;Anal. Chem. 2020, 92(1): 782-791)

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